Synthetic Biology | Microbiome
Robinson CM, Carreño D, Weber T, Chen Y and Riglar DT©. 2023. A discovery platform to identify inducible synthetic circuitry from varied microbial sources. 2023. bioRxiv. DOI: 2023.10.13.562223. Open access link.
Ramlaul K, Feng Z, Canavan C, de Martín Garrido N, Carreño D, Crone M, Jensen KE, Li B, Barnet H, Riglar DT, Freemont PS, Miller D and Aylett CHS. 2023. On-grid purification of electron microscopy samples via a 3D-printed flow-cell. J Struct Biol. 215 (3), 107999. Open access link.
Robinson CM, Short SE and Riglar DT©. 2022. Achieving spatially precise diagnosis and therapy in the mammalian gut using synthetic microbial gene circuits. Front Bioeng. Biotechnol 10. DOI: 10.3389/fbioe.2022.959441. Open access link.
Riglar DT, Richmond DL, Potvin-Trottier L, Verdegaal AA, Naydich AD, Bakshi S, Leoncini E, Paulsson J & Silver PA. Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator. Nat Commun 10, 4665 (2019). Open access link
[A preprint of this work was deposited as doi: https://doi.org/10.1101/472720].
Ziesack M, Gibson T, Shumaker AM, Oliver JKW, Riglar DT, Giessen TW, DiBenedetto NV, Lall K, Hsu BB, Bry L, Way JC, Silver PA and Gerber GK. Inducible cooperation in a synthetic gut bacterial consortium introduces population balance and stability. mSystems 4, e00352-19 (2019). Open access link
[A preprint of this work was deposited as doi: https://doi.org/10.1101/426171]
Naydich AD, Nangle SN, Bues JJ, Trivedi D, Nissar N, Inniss MC, Neiderhuber MJ, Way JC, Silver, PA* & Riglar DT*. Synthetic gene circuits enable systems-level biosensor trigger discovery at the host-microbiome interface. mSystems 4, e00125-19. Open access link. (* = Authors contributed equally to this work)
[A preprint of this work was deposited as doi: https://doi.org/10.1101/557934].
Riglar DT and Silver PA. Engineering bacteria for diagnostic and therapeutic applications. Nat Rev Microbiol, 16, 214-225. (2018). Open Access Link | Journal Link
Riglar DT, Giessen TW. Baym M, Kerns SJ, Niederhuber MJ, Bronson RT, Kotula JW, Gerber GK, Way JC and Silver PA. Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation. Nat Biotechnol 35, 653-658. Open Access Link.
[A preprint of this work was deposited as doi: https://doi.org/10.1101/075051]
[News & Views/ Commentary: Sedlmayer & Fussenegger, 2017, Nat Biomed Eng; Cell Systems Call, June 28 2017]
Malaria | Super-resolution Microscopy
Bullen HA, Sanders PR, Dans MG, Jonsdottir TK, Riglar DT, Looker O, Palmer CS, Kouskousis B, Charnaud SC, Triglia T, Gabriela M, Parkyn Schneider M, Chan J, de Koning-Ward TF, Baum J, Kazura JW, Beeson JG, Cowman AF, Gilson PR and Crabb BS. 2022. The Plasmodium falciparum parasitopherous vacuole protein P113 interacts with the parasite protein export machinery and maintains normal vacuole architecture. Mol Microbiol 117, 1245-1262. Open access link.
Baum J, Richard D and Riglar DT. 2017. Malaria parasite invasion: achieving superb resolution. Cell Host Microbe, 21, 294-296.
Riglar DT, Whitehead L, Cowman AF Rogers KL, Baum J. 2016. Localization-based imaging of malarial antigens during red cell entry reaffirms role for AMA1 but not MTRAP in invasion. J Cell Science,129, 228-242.
Olshina MA, Angrisano F, Marapana DS, Riglar DT, Bane K, Wong W, Catimel B, Yin MX, Holmes AB, Frischknecht F, Kovar DR and Baum J. 2015. Plasmodium falciparum coronin organizes arrays of parallel actin filaments potentially guiding directional motility in invasive malaria parasites. Malar J. 14, 280.
Dasgupta S, Auth T, Gov NS, Satchwell TJ, Hanssen E, Zuccala ES, Riglar DT, Toye AM, Betz T, Baum J and Gompper G. 2014. Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte. Biophys J, 107, 43-54.
Healer J, Thompson J, Riglar DT, Wilson DW, Yu-H C, Miura K, Chen L, Hodder A, Long C, Hansen D, Baum J and Cowman AF. 2013. Vaccination with Conserved Regions of Erythrocyte-Binding Antigens Induces Neutralizing Antibodies Against Multiple Strains of Plasmodium falciparum. PLoS ONE, 8, e72504.
Hanssen E*, Dekiwadia C*, Riglar DT, Rug M, Lemgruber L, Cowman AF, Cyrklaff M, Kudryashev M, Frischknecht F, Baum J and Ralph SA. 2013. Tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion. Cell Microbiol,15, 1457-1472. (* = authors contributed equally to this work). [Editor’s Choice article].
Lemgruber L*, Kudryashev M, Dekiwadia C, Riglar DT, Baum J, Stahlberg H, Ralph SA and Frischknecht F. 2013. Cryo-electron tomography reveals four-membrane architecture of the Plasmodium apicoplast. Malar J 12, 25. ( = authors contributed equally to this work).
Riglar DT, Rogers KL, Hanssen E, Turnbull L, Bullen HE, Charnaud SC, Przyborski J, Gilson PR, Whitchurch CB, Crabb BS, Baum J and Cowman AF. 2013. Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes. Nat Commun 4, 1415.
Baum J and Riglar DT. Imaging of the blood stage Plasmodium falciparum merozoite during erythrocyte invasion. In Moll K, Kaneko A, Scherf A and Wahlgren M (Eds.), Methods in Malaria Research 6th Ed. Glasgow: EVIMalaR and Manassas, VA: MR4/ATCC. (2013)
Riglar DT and Baum J. Static and dynamic imaging of erythrocyte invasion and early intraerythrocytic development in Plasmodium falciparum. Methods Mol Biol 923, 269-280. (2013)
Zuccala ES, Gout AM, Dekiwadia C, Marapana DS, Angrisano F, Turnbull L, Riglar DT, Rogers KL, Whitchurch CB, Ralph SA, Speed TP, and Baum J. 2012. Subcompartmentalisation of proteins in the rhoptries correlates with ordered events of erythrocyte invasion by the blood stage malaria parasite. PLoS ONE 7, e46160.
Bullen HE, Charnaud SC, Kalanon M, Riglar DT, Dekiwadia C, Torii M, Tsuboi T, Baum J, Ralph SA, Cowman AF, de Koning-Ward TF, Crabb BS and Gilson PR. 2012. Biosynthesis, localisation and macromolecular arrangement of the Plasmodium falciparum translocon of exported proteins; PTEX.J Biol Chem.287, 7871-7884.
Angrisano F, Riglar DT*, Sturm A*, Volz JC*, Delves MJ, Zuccala ES, Turnbull L, Dekiwadia C, Olshina MA, Marapana DS, Wong W, Mollard V, Bradin CH, Tonkin CJ, Gunning PW, Ralph SA, Whitchurch CB, Sinden RE, Cowman AF, McFadden GI and Baum J. 2012. Spatial localisation of actin filaments across developmental stages of the malaria parasite. PLoS ONE.7, e32188. (* = authors contributed equally to this work)
Chen L, Lopaticki S, Riglar DT, Dekiwadia C, Uboldi AD, Tham W-H, O’Neill MT, Richard D, Baum J, Ralph SA and Cowman AF. 2011. An EGF-like protein that forms a complex with PfRh5 and is required for invasion of human erythrocytes by Plasmodium falciparum. PLoS Pathog. 7, Article e1002199.
Wong W, Skau C, Marapana D, Hanssen E, Taylor N, Riglar DT, Zuccala E , Angrisano F, Lewis H, Catimel B, Clarke O , Kershaw N, Perugini M, Kovar D, Gulbis J and Baum J. 2011. Minimal requirements for actin filament disassembly revealed by structural analysis of malaria parasite Actin Depolymerizing Factor 1. Proc Natl Acad Sci USA. 24, 9869-9874.
Triglia T, Chen L, Lopaticki S, Dekiwadia C, Riglar DT, Hodder AN, Ralph SA, Baum J and Cowman AF. 2011. Plasmodium falciparum merozoite invasion is inhibited by antibodies that target the PfRh2a and b binding domains. PLoS Pathog. 6, Article e1002075.
Riglar DT*, Richard D*, Wilson DW^, Boyle MJ^, Dekiwadia C, Turnbull L, Angrisano F, Marapana DS, Rogers KL, Whitchurch CB, Beeson JG, Cowman AF, Ralph SA and Baum J. 2011. Super-resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte. Cell Host Microbe. 9, 9-20. [10-year anniversary feature article; Best of 2011 article] (*, ^ = authors contributed equally to this work)
Boyle MJ*, Wison DW*, Richards JS^, Riglar DT^, Tetteh KKA, Conway DJ, Ralph SA, Baum J and Beeson JG. 2010. Isolation of viable Plasmodium falciparum merozoites to define erythrocyte invasion events and advance vaccine and drug development. Proc Natl Acad Sci USA. 107, 14378-14383. (*, ^ = authors contributed equally to this work).
Richard D, Macraild CA, Riglar DT, Chan JA, Foley M, Baum J, Ralph SA, Norton RS and Cowman AF. 2010. Interaction between plasmodium falciparum apical membrane antigen 1 and the Rhoptry neck protein complex defines a key step in the erythrocyte invasion process of malaria parasites. J Biol Chem. 285, 14815-14822.
Other publications, articles and blog posts:
Future of Research Board of Directors, McDowell GS. 2016. Future of Research Response to NIGMS Request for Information (RFI): Strategies for Modernizing Biomedical Graduate Education (NOT-GM-16-109). The Winnower 3, e147071.15403.
Riglar D. 2016. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux, Addgene Blog.
Riglar D. 2015. Advocating for changes to science at Future of Research Boston, Scitable by Nature Education.
Riglar D. 2015. The Future of Research Symposium Boston, Addgene Blog.
Riglar D and Hilton D. 2014. Voices of a generation: young scientists must be seen and heard, The Conversation Australia.
Riglar Dand Tovkach A. 2012. Meeting Report: Science Meets Parliament. Australian Biochemist. 43 (3).
Riglar D. 2012. Freezing parasites in the European Sun. Australian Biochemist. 43(2).
Ehlgen F and Riglar D. 2010. Meeting Report: Practical Course in imaging and microscopy. Biotechnol J 5, 1253-1256.
Fletcher JI, Meusburger S, Hawkins CJ, Riglar DT,Lee EF, Fairlie WD, Huang DCS and Adams, JM. 2008. Apoptosis is triggered when prosurvival Bcl-2 proteins cannot restrain Bax. Proc Natl Acad Sci USA. 105,18081 – 18087.